Follow the double centrifuge technique below to achieve platelet-poor plasma.
1. Spin down the specimen at 3000 RPM (1600g) for a full 10 minutes.
2. Transfer the plasma to a plastic tube with a plastic pipet, staying clear from the buffy coat layer. Do not use glass tubes or glass Pasteur pipets as glass can activate the clotting cascade.
3. Spin down the plasma portion again at 3000 RPM for 10 minutes. With another plastic pipet, transfer the plasma to another plastic tube, staying clear of the bottom portion of the tube where the platelets lie.
4. Freeze the sample. The sample must remain frozen in transit. Ship on dry ice.